SARS-CoV-2 serology: Validation of high-throughput chemiluminescent immunoassay (CLIA) platforms and a field study in British Columbia.

ABSTRACT:

Background

SARS-CoV-2 antibody testing is required for estimating population seroprevalence and vaccine response studies. It may also increase case identification when used as an adjunct to routine molecular testing. We performed a validation study and evaluated the use of automated high-throughput assays in a field study of COVID-19-affected care facilities.

Methods

Six automated assays were assessed: 1) DiaSorin LIAISON^TM SARS-CoV-2 S1/S2 IgG; 2) Abbott ARCHITECT^TM SARS-CoV-2 IgG; 3) Ortho VITROS^TM Anti-SARS-CoV-2 Total; 4) VITROS^TM Anti-SARS-CoV-2 IgG; 5) Siemens SARS-CoV-2 Total Assay; and 6) Roche Elecsys^TM Anti-SARS-CoV-2. The validation study included 107 samples (42 known positive; 65 presumed negative). The fiel

SUBMITTER: Sekirov I 

PROVIDER: S-EPMC8282439 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature