Single-Cell Characterization of the Immune Microenvironment of Melanoma Brain and Leptomeningeal Metastases.

ABSTRACT:

Purpose

Melanoma brain metastases (MBM) and leptomeningeal melanoma metastases (LMM) are two different manifestations of melanoma CNS metastasis. Here, we used single-cell RNA sequencing (scRNA-seq) to define the immune landscape of MBM, LMM, and melanoma skin metastases.

Experimental design

scRNA-seq was undertaken on 43 patient specimens, including 8 skin metastases, 14 MBM, and 19 serial LMM specimens. Detailed cell type curation was performed, the immune landscapes were mapped, and key results were validated by IHC and flow cytometry. Association analyses were undertaken to identify immune cell subsets correlated with overall survival.

Results

The LMM microenvironment was characterized by an immune-suppressed T-cell landscape distinct from that of brain and skin m

SUBMITTER: Smalley I 

PROVIDER: S-EPMC8282775 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature