The α2δ-1/NMDA receptor complex is involved in brain injury after intracerebral hemorrhage in mice.

ABSTRACT:

Background

Intracerebral hemorrhage (ICH), a common cerebrovascular disease, seriously threatens human health and has severe secondary injuries, while existing treatment methods have many limitations. α2δ-1 is a subunit of voltage-gated Ca²⁺ channels (VGCCs) and can act on glutamate receptor N-methyl-D-aspartate receptors (NMDARs) to relieve neuropathic pain.

Methods

We first performed ICH modeling on WT mice and Cacna2d1 knockout (KO) mice. The expression levels of GluN1 and α2δ-1 were measured by Western blot and q-PCR, and the interaction between the two proteins was evaluated by co-precipitation. The neuronal apoptosis was detected by the TUNEL assay, and the expression levels of inflammatory factors were assessed by ELISA. The nerve functions of mice were eva

SUBMITTER: Li J 

PROVIDER: S-EPMC8283164 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature