Role of Free Radical Formation in Murine Cytomegalovirus-Induced Hearing Loss.

ABSTRACT:

Objectives

The goal of the study was to determine whether reactive oxygen species (ROS) mediates cytomegalovirus (CMV)-induced labyrinthitis.

Study design

Murine model of CMV infection.

Setting

University of Utah laboratory.

Subjects and methods

Nrf2 knockout mice were inoculated with murine CMV. Auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAEs) were then performed on these and uninfected controls. BALB/c mice were inoculated with murine CMV to determine whether a marker for ROS production, dihydroethidium (DHE), is expressed 7 days after inoculation. Finally, 2 antioxidants-D-methionine and ACE-Mg (vitamins A, C, and E with magnesium)-were administered 1 hour before and after infection in inoculated mice for 14 days. Tempo

SUBMITTER: Pecha PP 

PROVIDER: S-EPMC8283710 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature