Apramycin resistance in epidemic carbapenem-resistant Klebsiella pneumoniae ST258 strains.

ABSTRACT:

Background

Recent studies indicated that the monosubstituted deoxystreptamine aminoglycoside apramycin is a potent antibiotic against a wide range of MDR Gram-negative pathogens.

Objectives

To evaluate the in vitro activity of apramycin against carbapenem-resistant Klebsiella pneumoniae (CRKp) isolates from New York and New Jersey, and to explore mechanisms of apramycin resistance.

Methods

Apramycin MICs were determined by broth microdilution for 155 CRKp bloodstream isolates collected from 2013 to 2018. MLST STs, wzi capsular types and apramycin resistance gene aac(3')-IV were examined by PCR and Sanger sequencing. Selected isolates were further characterized by conjugation experiments and WGS.

Results

Apramycin MIC50/90 values were 8 and >128 mg/L for CRKp is

SUBMITTER: Hao M 

PROVIDER: S-EPMC8283726 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature