Alcohol-associated intestinal dysbiosis alters mucosal-associated invariant T-cell phenotype and function.

ABSTRACT:

Background

Chronic alcohol consumption is associated with a compromised innate and adaptive immune responses to infectious disease. Mucosa-associated invariant T (MAIT) cells play a critical role in antibacterial host defense. However, whether alcohol-associated deficits in innate and adaptive immune responses are mediated by alterations in MAIT cells remains unclear.

Methods

To investigate the impact of alcohol on MAIT cells, mice were treated with binge-on-chronic alcohol for 10 days and sacrificed at day 11. MAIT cells in the barrier organs (lung, liver, and intestine) were characterized by flow cytometry. Two additional sets of animals were used to examine the involvement of gut microbiota on alcohol-induced MAIT cell changes: (1) Cecal microbiota from alcohol-fed (AF) m

SUBMITTER: Gu M 

PROVIDER: S-EPMC8283808 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature