Assessment of changes in autophagic vesicles in human immune cell lines exposed to nano particles.

ABSTRACT:

Background

Safe and rational development of nanomaterials for clinical translation requires the assessment of potential biocompatibility. Autophagy, a critical homeostatic pathway intrinsically linked to cellular health and inflammation, has been shown to be affected by nanomaterials. It is, therefore, important to be able to assess possible interactions of nanomaterials with autophagic processes.

Results

CEM (T cell), Raji (B lymphocyte), and THP-1 (human monocyte) cell lines were subject to treatment with rapamycin and chloroquine, known to affect the autophagic process, in order to evaluate cell line-specific responses. Flow cytometric quantification of a fluorescent autophagic vacuole stain showed that maximum observable effects (105%, 446%, and 149% of negative controls

SUBMITTER: David CAW 

PROVIDER: S-EPMC8283997 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature