A multi-country study using MALDI-TOF mass spectrometry for rapid identification of Burkholderia pseudomallei.

ABSTRACT:

Background

Burkholderia pseudomallei is the bacterial causative agent of melioidosis, a difficult disease to diagnose clinically with high mortality if not appropriately treated. Definitive diagnosis requires isolation and identification of the organism. With the increased adoption of MALDI-TOF MS for the identification of bacteria, we established a method for rapid identification of B. pseudomallei using the Vitek MS, a system that does not currently have B. pseudomallei in its in-vitro diagnostic database.

Results

A routine direct spotting method was employed to create spectra and SuperSpectra. An initial B. pseudomallei SuperSpectrum was created at Shoklo Malaria Research Unit (SMRU) from 17 reference isolates (46 spectra). When tested, this initial SMRU SuperSpectrum was

SUBMITTER: Watthanaworawit W 

PROVIDER: S-EPMC8283998 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature