Dataset Information

Identification of a Novel Plasmid-Mediated Carbapenemase-Encoding Gene, bla_VMB-2, in Vibrio diabolicus.

ABSTRACT: Here, we characterized a carbapenem-resistant Vibrio diabolicus strain of shrimp origin with various experiments and bioinformatics analysis. A novel metallo-β-lactamase (MBL) gene, bla_VMB-2, that confers resistance to β-lactams, including meropenem and cephalosporins, was identified on a plasmid-borne composite transposon, ISShfr9-ISCR1-bla_VMB-2-bla_CARB-12-aadA1-ISShfr9, which is capable of generating a bla_VMB-2-bearing circular intermediate. ISShfr9 was found to be disseminated in multidrug-resistant (MDR) pathogens, arousing the concern of further transmission of a bla_VMB-2-bearing circular intermediate to clinical Enterobacterales strains via such insertion sequences, which warrants further investigations.

SUBMITTER: Liu M

PROVIDER: S-EPMC8284434 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Publications

Identification of a Novel Plasmid-Mediated Carbapenemase-Encoding Gene, blaVMB-2, in Vibrio diabolicus.

Liu Ming M Zhang Wenhui W Peng Kai K Wang Zhiqiang Z Li Ruichao R

Antimicrobial agents and chemotherapy 20210716 8

Here, we characterized a carbapenem-resistant Vibrio diabolicus strain of shrimp origin with various experiments and bioinformatics analysis. A novel metallo-β-lactamase (MBL) gene, blaVMB-2, that confers resistance to β-lactams, including meropenem and cephalosporins, was identified on a plasmid-borne composite transposon, ISShfr9-ISCR1-blaVMB-2-blaCARB-12-aadA1-ISShfr9, which is capable of generating a bla ...[more]

PMID: 34097496

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Project description:This study characterizes a new genetic structure containing a multicopy of a blaVIM-2 variant with an A676C substitution, blaVIM-63. This gene was detected on the chromosome of two carbapenem-resistant clinical strains of Citrobacter freundii ST22 recovered from two patients, separated by a 6-month period, and previously in Pseudomonas aeruginosa ST2242 from the same hospital unit. Short-read sequencing was used to characterize the new variant in both species, and long-read sequencing was used to characterize the genome of C. freundii. On the P. aeruginosa chromosome, the blaVIM-63 gene was inserted between ISPsy 42-type sequences, flanked by an intl1 sequence, nearby aph(3')-VI, and sul1. On the C. freundii chromosome, the blaVIM-63 gene was inserted into a Tn6230-like transposon as a stable five-tandem-repeat multimer, flanked by the same intl1 as in P. aeruginosa. This structure was stable across subcultures and did not change in the presence of carbapenems. The blaVIM-63 gene was cloned into the pCR-Blunt plasmid to study antimicrobial susceptibility patterns and into pET29a for kinetic activity analysis. VIM-63 showed higher Km values than VIM-2 for ceftazidime and cefepime and higher kcat values for cefotaxime, ceftazidime, imipenem, and ertapenem, without differences in MIC values. This is the first study to describe this new variant, VIM-63, in two different species with a chromosomal location integrated into different mobile elements and the first to describe a stable multimer of a metallo-β-lactamase. Despite the amino acid substitution, the susceptibility pattern of the new variant was similar to that of VIM-2. IMPORTANCE VIM group metallo-β-lactamases are usually captured by IntI1 integrases. This work describes the detection for the first time of a novel, previously unknown variant of VIM-2, VIM-63. This carbapenemase has been found on the chromosome of two different species, Citrobacter freundii and Pseudomonas aeruginosa, from the same hospital. The adjacent genetic environment of the blaVIM-63 gene would indicate that the capture of this gene by IntI1 has occurred in two different genetic events in each of the species, and in one there has been a stable integration of tandem copies of this gene.

Dataset Information

Identification of a Novel Plasmid-Mediated Carbapenemase-Encoding Gene, bla_VMB-2, in Vibrio diabolicus.

Publications

Identification of a Novel Plasmid-Mediated Carbapenemase-Encoding Gene, <i>bla</i><sub>VMB-2</sub>, in Vibrio diabolicus.

Similar Datasets

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