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Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage.

ABSTRACT: Aging is associated with dysregulated immune functions. Here, we investigated the impact of age on neutrophil diapedesis. Using confocal intravital microscopy, we found that in aged mice, neutrophils adhered to vascular endothelium in inflamed tissues but exhibited a high frequency of reverse transendothelial migration (rTEM). This retrograde breaching of the endothelium by neutrophils was governed by enhanced production of the chemokine CXCL1 from mast cells that localized at endothelial cell (EC) junctions. Increased EC expression of the atypical chemokine receptor 1 (ACKR1) supported this pro-inflammatory milieu in aged venules. Accumulation of CXCL1 caused desensitization of the chemokine receptor CXCR2 on neutrophils and loss of neutrophil directional motility within EC junctions. Fluorescent tracking revealed that in aged mice, neutrophils undergoing rTEM re-entered the circulation and disseminated to the lungs where they caused vascular leakage. Thus, neutrophils stemming from a local inflammatory site contribute to remote organ damage, with implication to the dysregulated systemic inflammation associated with aging.

SUBMITTER: Barkaway A

PROVIDER: S-EPMC8284598 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage.

Barkaway Anna A Rolas Loïc L Joulia Régis R Bodkin Jennifer J Lenn Tchern T Owen-Woods Charlotte C Reglero-Real Natalia N Stein Monja M Vázquez-Martínez Laura L Girbl Tamara T Poston Robin N RN Golding Matthew M Saleeb Rebecca S RS Thiriot Aude A von Andrian Ulrich H UH Duchene Johan J Voisin Mathieu-Benoit MB Bishop Cleo L CL Voehringer David D Roers Axel A Rot Antal A Lämmermann Tim T Nourshargh Sussan S

Immunity 20210524 7

Aging is associated with dysregulated immune functions. Here, we investigated the impact of age on neutrophil diapedesis. Using confocal intravital microscopy, we found that in aged mice, neutrophils adhered to vascular endothelium in inflamed tissues but exhibited a high frequency of reverse transendothelial migration (rTEM). This retrograde breaching of the endothelium by neutrophils was governed by enhanced production of the chemokine CXCL1 from mast cells that localized at endothelial cell ( ...[more]

PMID: 34033752

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Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage.

Publications

Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage.

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