A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development.

ABSTRACT: Biomechanical forces intimately contribute to cardiac morphogenesis. However, volumetric imaging to investigate the cardiac mechanics with high temporal and spatial resolution remains an imaging challenge. We hereby integrated light-field microscopy (LFM) with light-sheet fluorescence microscopy (LSFM), coupled with a retrospective gating method, to simultaneously access myocardial contraction and intracardiac blood flow at 200 volumes per second. While LSFM allows for the reconstruction of the myocardial function, LFM enables instantaneous acquisition of the intracardiac blood cells traversing across the valves. We further adopted deformable image registration to quantify the ventricular wall displacement and particle tracking velocimetry to monitor intracardiac blood flow. The integratio

SUBMITTER: Wang Z 

PROVIDER: S-EPMC8284633 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature