Mutants of human ACE2 differentially promote SARS-CoV and SARS-CoV-2 spike mediated infection.

ABSTRACT: SARS-CoV and SARS-CoV-2 encode spike proteins that bind human ACE2 on the cell surface to enter target cells during infection. A small fraction of humans encode variants of ACE2, thus altering the biochemical properties at the protein interaction interface. These and other ACE2 coding mutants can reveal how the spike proteins of each virus may differentially engage the ACE2 protein surface during infection. We created an engineered HEK 293T cell line for facile stable transgenic modification, and expressed the major human ACE2 allele or 28 of its missense mutants, 24 of which are possible through single nucleotide changes from the human reference sequence. Infection with SARS-CoV or SARS-CoV-2 spike pseudotyped lentiviruses revealed that high ACE2 cell-surface expression could mask the eff

SUBMITTER: Shukla N 

PROVIDER: S-EPMC8284657 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature