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Rampant prophage movement among transient competitors drives rapid adaptation during infection.

ABSTRACT: Interactions between bacteria, their close competitors, and viral parasites are common in infections, but understanding of these eco-evolutionary dynamics is limited. Most examples of adaptations caused by phage lysogeny are through the acquisition of new genes. However, integrated prophages can also insert into functional genes and impart a fitness benefit by disrupting their expression, a process called active lysogeny. Here, we show that active lysogeny can fuel rapid, parallel adaptations in establishing a chronic infection. These recombination events repeatedly disrupted genes encoding global regulators, leading to increased cyclic di-GMP levels and elevated biofilm production. The implications of prophage-mediated adaptation are broad, as even transient members of microbial communities can alter the course of evolution and generate persistent phenotypes associated with poor clinical outcomes.

SUBMITTER: Marshall CW

PROVIDER: S-EPMC8284892 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Rampant prophage movement among transient competitors drives rapid adaptation during infection.

Marshall Christopher W CW Gloag Erin S ES Lim Christina C Wozniak Daniel J DJ Cooper Vaughn S VS

Science advances 20210716 29

Interactions between bacteria, their close competitors, and viral parasites are common in infections, but understanding of these eco-evolutionary dynamics is limited. Most examples of adaptations caused by phage lysogeny are through the acquisition of new genes. However, integrated prophages can also insert into functional genes and impart a fitness benefit by disrupting their expression, a process called active lysogeny. Here, we show that active lysogeny can fuel rapid, parallel adaptations in ...[more]

PMID: 34272240

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