Charge transfer dissociation of a branched glycan with alkali and alkaline earth metal adducts.

ABSTRACT: Alkali and alkaline earth metal adducts of a branched glycan, XXXG, were analyzed with helium charge transfer dissociation (He-CTD) and low-energy collision-induced dissociation (LE-CID) to investigate if metalation would impact the type of fragments generated and the structural characterization of the analyte. The studied adducts included 1+ and 2+ precursors involving one or more of the cations: H⁺ , Na⁺ , K⁺ , Ca²⁺ , and Mg²⁺ . Regardless of the metal adduct, He-CTD generated abundant and numerous glycosidic and cross-ring cleavages that were structurally informative and able to identify the 1,4-linkage and 1,6-branching patterns. In contrast, the LE-CID spectra mainly contained glycosidic cleavages, consecutive fragments, and numer

SUBMITTER: Sasiene ZJ 

PROVIDER: S-EPMC8285033 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature