Reconciling functional differences in populations of neurons recorded with two-photon imaging and electrophysiology.

ABSTRACT: Extracellular electrophysiology and two-photon calcium imaging are widely used methods for measuring physiological activity with single-cell resolution across large populations of cortical neurons. While each of these two modalities has distinct advantages and disadvantages, neither provides complete, unbiased information about the underlying neural population. Here, we compare evoked responses in visual cortex recorded in awake mice under highly standardized conditions using either imaging of genetically expressed GCaMP6f or electrophysiology with silicon probes. Across all stimulus conditions tested, we observe a larger fraction of responsive neurons in electrophysiology and higher stimulus selectivity in calcium imaging, which was partially reconciled by applying a spikes-to-calcium for

SUBMITTER: Siegle JH 

PROVIDER: S-EPMC8285106 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature