Compartmentalization-aided interaction screening reveals extensive high-order complexes within the SARS-CoV-2 proteome.

ABSTRACT: Bearing a relatively large single-stranded RNA genome in nature, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes sophisticated replication/transcription complexes (RTCs), mainly composed of a network of nonstructural proteins and nucleocapsid protein, to establish efficient infection. In this study, we develop an innovative interaction screening strategy based on phase separation in cellulo, namely compartmentalization of protein-protein interactions in cells (CoPIC). Utilizing CoPIC screening, we map the interaction network among RTC-related viral proteins. We identify a total of 47 binary interactions among 14 proteins governing replication, discontinuous transcription, and translation of coronaviruses. Further exploration via CoPIC leads to the discovery of extensi

SUBMITTER: Xu W 

PROVIDER: S-EPMC8285250 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature