Continuous process technology for glucoside production from sucrose using a whole cell-derived solid catalyst of sucrose phosphorylase.

ABSTRACT: Advanced biotransformation processes typically involve the upstream processing part performed continuously and interlinked tightly with the product isolation. Key in their development is a catalyst that is highly active, operationally robust, conveniently produced, and recyclable. A promising strategy to obtain such catalyst is to encapsulate enzymes as permeabilized whole cells in porous polymer materials. Here, we show immobilization of the sucrose phosphorylase from Bifidobacterium adolescentis (P134Q-variant) by encapsulating the corresponding E. coli cells into polyacrylamide. Applying the solid catalyst, we demonstrate continuous production of the commercial extremolyte 2-α-D-glucosyl-glycerol (2-GG) from sucrose and glycerol. The solid catalyst exhibited similar activity (≥70%) as t

SUBMITTER: Kruschitz A 

PROVIDER: S-EPMC8285329 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature