The chromatin, topological and regulatory properties of pluripotency-associated poised enhancers are conserved in vivo.

ABSTRACT: Poised enhancers (PEs) represent a genetically distinct set of distal regulatory elements that control the expression of major developmental genes. Before becoming activated in differentiating cells, PEs are already bookmarked in pluripotent cells with unique chromatin and topological features that could contribute to their privileged regulatory properties. However, since PEs were originally characterized in embryonic stem cells (ESC), it is currently unknown whether PEs are functionally conserved in vivo. Here, we show that the chromatin and 3D structural features of PEs are conserved among mouse pluripotent cells both in vitro and in vivo. We also uncovered that the interactions between PEs and their target genes are globally controlled by the combined action of Polycomb, Trithorax and a

SUBMITTER: Crispatzu G 

PROVIDER: S-EPMC8285398 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature