Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing.

ABSTRACT: Cytosine or adenine base editors (CBEs or ABEs) hold great promise in therapeutic applications because they enable the precise conversion of targeted base changes without generating of double-strand breaks. However, both CBEs and ABEs induce substantial off-target DNA editing, and extensive off-target RNA single nucleotide variations in transfected cells. Therefore, the potential effects of deaminases induced by DNA base editors are of great importance for their clinical applicability. Here, the transcriptome-wide deaminase effects on gene expression and splicing is examined. Differentially expressed genes (DEGs) and differential alternative splicing (DAS) events, induced by base editors, are identified. Both CBEs and ABEs generated thousands of DEGs and hundreds of DAS events. For enginee

SUBMITTER: Fan J 

PROVIDER: S-EPMC8285404 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature