TRPV4-Rho signaling drives cytoskeletal and focal adhesion remodeling in trabecular meshwork cells.

ABSTRACT: Intraocular pressure (IOP) is dynamically regulated by the trabecular meshwork (TM), a mechanosensitive tissue that protects the eye from injury through dynamic regulation of aqueous humor flow. TM compensates for mechanical stress impelled by chronic IOP elevations through increased actin polymerization, tissue stiffness, and contractility. This process has been associated with open angle glaucoma; however, the mechanisms that link mechanical stress to pathological cytoskeletal remodeling downstream from the mechanotransducers remain poorly understood. We used fluorescence imaging and biochemical analyses to investigate cytoskeletal and focal adhesion remodeling in human TM cells stimulated with physiological strains. Mechanical stretch promoted F-actin polymerization, increased the numbe

SUBMITTER: Lakk M 

PROVIDER: S-EPMC8285634 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature