Lung branching morphogenesis is accompanied by temporal metabolic changes towards a glycolytic preference.

ABSTRACT:

Background

Lung branching morphogenesis is characterized by epithelial-mesenchymal interactions that ultimately define the airway conducting system. Throughout this process, energy and structural macromolecules are necessary to sustain the high proliferative rates. The extensive knowledge of the molecular mechanisms underlying pulmonary development contrasts with the lack of data regarding the embryonic lung metabolic requirements. Here, we studied the metabolic profile associated with the early stages of chicken pulmonary branching.

Methods

In this study, we used an ex vivo lung explant culture system and analyzed the consumption/production of extracellular metabolic intermediates associated with glucose catabolism (alanine, lactate, and acetate) by ¹H-NMR spectr

SUBMITTER: Fernandes-Silva H 

PROVIDER: S-EPMC8285861 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature