Deploying new generation sequencing for the study of flesh color depletion in Atlantic Salmon (Salmo salar).

ABSTRACT:

Background

The flesh pigmentation of farmed Atlantic salmon is formed by accumulation of carotenoids derived from commercial diets. In the salmon gastrointestinal system, the hindgut is considered critical in the processes of carotenoids uptake and metabolism. In Tasmania, flesh color depletion can noticeably affect farmed Atlantic salmon at different levels of severity following extremely hot summers. In this study, RNA sequencing (RNA-Seq) was performed to investigate the reduction in flesh pigmentation. Library preparation is a key step that significantly impacts the effectiveness of RNA sequencing (RNA-Seq) experiments. Besides the commonly used whole transcript RNA-Seq method, the 3' mRNA-Seq method is being applied widely, owing to its reduced cost, enabling more repeats to b

SUBMITTER: Vo TTM 

PROVIDER: S-EPMC8285899 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature