Bidirectional flow of the funny current (If) during the pacemaking cycle in murine sinoatrial node myocytes.

ABSTRACT: Sinoatrial node myocytes (SAMs) act as cardiac pacemaker cells by firing spontaneous action potentials (APs) that initiate each heartbeat. The funny current (If) is critical for the generation of these spontaneous APs; however, its precise role during the pacemaking cycle remains unresolved. Here, we used the AP-clamp technique to quantify If during the cardiac cycle in mouse SAMs. We found that If is persistently active throughout the sinoatrial AP, with surprisingly little voltage-dependent gating. As a consequence, it carries both inward and outward current around its reversal potential of -30 mV. Despite operating at only 2 to 5% of its maximal conductance, If carries a substantial fraction of both depolarizing and repolarizing net charge movement during the firing cycle. We also show

SUBMITTER: Peters CH 

PROVIDER: S-EPMC8285948 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature