Abnormal Macrophage Polarization in Patients with Myelodysplastic Syndrome.

ABSTRACT:

Background

This study is aimed at assessing the subsets of bone marrow macrophages in patients with myelodysplastic syndrome (MDS) and exploring the role of macrophages in the pathogenesis of MDS.

Methods

Thirty-eight newly diagnosed MDS patients were enrolled in the Department of Hematology of General Hospital of Tianjin Medical University from June 2015 to June 2016. Bone marrow monocytes and macrophage subsets (M1/M2) were detected in patients with MDS and normal controls by flow cytometry. M1 macrophages were cultured in vitro, and the expression of IL-1β and TNF-α mRNA was measured using real-time polymerase chain reaction.

Results

Compared with the normal control group, the proportion of bone marrow monocytes was higher (2.11 ± 0.93% vs. 3.6

SUBMITTER: Zhang G 

PROVIDER: S-EPMC8286189 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature