Fine Sampling of Sequence Space for Membrane Protein Structural Biology.

ABSTRACT: We describe an enhancement of traditional genomics-based approaches to improve the success of structure determination of membrane proteins. Following a broad screen of sequence space to identify initial expression-positive targets, we employ a second step to select orthologs with closely related sequences to these hits. We demonstrate that a greater percentage of these latter targets express well and are stable in detergent, increasing the likelihood of identifying candidates that will ultimately yield structural information.

SUBMITTER: Loukeris M 

PROVIDER: S-EPMC8286341 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature