¹⁸F-PEG1-Vinyl Sulfone-Labeled Red Blood Cells as Positron Emission Tomography Agent to Image Intra-Abdominal Bleeding.

ABSTRACT: ¹⁸F-Labeled blood pool agents (BPAs) have attracted great attention for identifying bleeding sites. However, many BPAs are not sufficiently evaluated partially due to the limitations of labeling methods. In our previous work, we noticed that ¹⁸F-PEG1-vinyl sulfone (¹⁸F-VS) could efficiently label red blood cells (RBCs) ex vivo and in situ. However, its application as BPA is not fully evaluated. In this study, we systematically explored the feasibility of using ¹⁸F-VS-labeled RBCs as a positron emission tomography (PET) BPA for intra-abdominal bleeding diagnosis. In brief, we first optimized the labeling conditions, which lead to an 80% labeling yield of RBCs after incubating with ¹⁸F-VS in phosphate-buffered saline (PBS)

SUBMITTER: Zhang X 

PROVIDER: S-EPMC8287037 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature