Proximity to the Promoter and Terminator Regions Regulates the Transcription Enhancement Potential of an Intron.

ABSTRACT: An evolutionarily conserved feature of introns is their ability to enhance expression of genes that harbor them. Introns have been shown to regulate gene expression at the transcription and post-transcription level. The general perception is that a promoter-proximal intron is most efficient in enhancing gene expression and the effect diminishes with the increase in distance from the promoter. Here we show that the intron regains its positive influence on gene expression when in proximity to the terminator. We inserted ACT1 intron into different positions within IMD4 and INO1 genes. Transcription Run-On (TRO) analysis revealed that the transcription of both IMD4 and INO1 was maximal in constructs with a promoter-proximal intron and decreased with the incre

SUBMITTER: Dwyer K 

PROVIDER: S-EPMC8287100 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature