Molecular detection and identification of Culex flavivirus in mosquito species from Jeju, Republic of Korea.

ABSTRACT:

Background

Mosquito-borne flaviviruses are prime pathogens and have been a major hazard to humans and animals. They comprise several arthropod-borne viruses, including dengue virus, yellow fever virus, Japanese encephalitis virus, and West Nile virus. Culex flavivirus (CxFV) is a member of the insect-specific flavivirus (ISF) group belonging to the genus Flavivirus, which is widely distributed in a variety of mosquito populations.

Methods

Viral nucleic acid was extracted from adult mosquito pools and subjected to reverse transcriptase nested polymerase chain reaction (PCR) using target-specific primers for detecting CxFV nonstructural protein 5 (NS5). The PCR-positive samples were then sequenced, and a phylogenetic tree was constructed, including reference sequences obtained

SUBMITTER: Chatterjee S 

PROVIDER: S-EPMC8287664 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature