Application of CRISPR/Cas9 technology in wild apple (Malus sieverii) for paired sites gene editing.

ABSTRACT:

Background

Xinjiang wild apple is an important tree of the Tianshan Mountains, and in recent years, it has undergone destruction by many biotic and abiotic stress and human activities. It is necessary to use new technologies to research its genomic function and molecular improvement. The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been successfully applied to genetic improvement in many crops, but its editing capability varies depending on the different combinations of the synthetic guide RNA (sgRNA) and Cas9 protein expression devices.

Results

In this study, we used 2 systems of vectors with paired sgRNAs targeting to MsPDS. As expected, we successfully induced the albino phenotype of calli and buds in both

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8287690 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature