Cell-free genomics reveal intrinsic, cooperative and competitive determinants of chromatin interactions.

ABSTRACT: Metazoan transcription factors distinguish their response elements from a large excess of similar sequences. We explored underlying principles of DNA shape read-out and factor cooperativity in chromatin using a unique experimental system. We reconstituted chromatin on Drosophila genomes in extracts of preblastoderm embryos, mimicking the naïve state of the zygotic genome prior to developmental transcription activation. We then compared the intrinsic binding specificities of three recombinant transcription factors, alone and in combination, with GA-rich recognition sequences genome-wide. For MSL2, all functional elements reside on the X chromosome, allowing to distinguish physiological elements from non-functional 'decoy' sites. The physiological binding profile of MSL2 is approximated thro

SUBMITTER: Eggers N 

PROVIDER: S-EPMC8287947 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature