Mycoplasma affects baseline gene expression and the response to glucocorticoids in vocal fold fibroblasts.

ABSTRACT: Introduction. In vitro experimentation is intentionally contrived to isolate specific phenomena in the context of profound biological complexity. Mycoplasmas in the upper airway likely contribute to this complexity and play a largely unknown role in both health and disease. Similarly, the presence and role of mycoplasma in in vitro investigation are largely unknown.Hypothesis. We hypothesize mycoplasma in human vocal fold fibroblasts (VFF) will affect both basal gene-expression patterns as well as the cell response to exogenous stimuli.Aim. We sought to determine mycoplasma presence across vocal fold fibroblast cultures, basal transcriptional changes as a function of mycoplasma, and responsiveness to exogenous glucocorticoids in mycoplasma-positive and -n

SUBMITTER: Doyle C 

PROVIDER: S-EPMC8289201 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature