V-pipe: a computational pipeline for assessing viral genetic diversity from high-throughput data.

ABSTRACT:

Motivation

High-throughput sequencing technologies are used increasingly not only in viral genomics research but also in clinical surveillance and diagnostics. These technologies facilitate the assessment of the genetic diversity in intra-host virus populations, which affects transmission, virulence and pathogenesis of viral infections. However, there are two major challenges in analysing viral diversity. First, amplification and sequencing errors confound the identification of true biological variants, and second, the large data volumes represent computational limitations.

Results

To support viral high-throughput sequencing studies, we developed V-pipe, a bioinformatics pipeline combining various state-of-the-art statistical models and computational tools for automated end-

SUBMITTER: Posada-Cespedes S 

PROVIDER: S-EPMC8289377 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature