Chloroplast and mitochondrial DNA editing in plants.

ABSTRACT: Plant organelles including mitochondria and chloroplasts contain their own genomes, which encode many genes essential for respiration and photosynthesis, respectively. Gene editing in plant organelles, an unmet need for plant genetics and biotechnology, has been hampered by the lack of appropriate tools for targeting DNA in these organelles. In this study, we developed a Golden Gate cloning system¹, composed of 16 expression plasmids (8 for the delivery of the resulting protein to mitochondria and the other 8 for delivery to chloroplasts) and 424 transcription activator-like effector subarray plasmids, to assemble DddA-derived cytosine base editor (DdCBE)² plasmids and used the resulting DdCBEs to efficiently promote point mutagenesis in mitochondria and chloroplasts.

SUBMITTER: Kang BC 

PROVIDER: S-EPMC8289734 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature