M2 macrophage-derived extracellular vesicles facilitate CD8+T cell exhaustion in hepatocellular carcinoma via the miR-21-5p/YOD1/YAP/β-catenin pathway.

ABSTRACT: Hepatocellular carcinoma (HCC) is a common malignancy. CD8⁺ T cell-mediated immune response is critical for the inhibition of HCC progression. M2 macrophages participate in HCC progression. This study set out to investigate the effect of M2 macrophage-derived extracellular vesicles (EVs) on CD8⁺ T cell exhaustion in HCC. M2 macrophage-derived EVs were isolated and identified. The murine model of primary HCC was established through DEN/CCl₄ induction, and model mice were injected with EVs. Peripheral blood mononuclear cells (PBMCs) were isolated from the mouse liver and CD8⁺ T cells were sorted. The expressions of immune checkpoint inhibitory receptors and effector cytokines on CD8⁺ T cells were detected, followed by the evaluation of C

SUBMITTER: Pu J 

PROVIDER: S-EPMC8289864 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature