Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow.

ABSTRACT: Disturbed blood flow (d-flow) has been known to induce changes of the cells in the arterial wall, increasing the risk of atherosclerosis. However, the heterogeneity of the vascular cell populations under d-flow remains less understood. To generate d-flow in vivo, partial carotid artery ligation (PCL) was performed. Seven days after ligation, single-cell RNA sequencing of nine left carotid arteries (LCA) from the PCL group (10,262 cells) or control group (14,580 cells) was applied and a single-cell atlas of gene expression was constructed. The integrated analysis identified 15 distinct carotid cell clusters, including 10 d-flow-relevant subpopulations. Among endothelial cells, at least four subpopulations were identified, including Klk8^hi ECs, Lrp1^hi ECs, Dkk2^hi</

SUBMITTER: Li F 

PROVIDER: S-EPMC8290019 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature