Assessment of Epigenetic and Phenotypic Variation in Populus nigra Regenerated via Sequential Regeneration.

ABSTRACT: Somatic variation has been demonstrated in tissue culture regenerated plants of many species. In the genus Populus, phenotypic variation caused by changes in 5-methylcytosine within the plant genome have been reported. To date, the phenotypic and epigenetic stability of plants regenerated from sequential regeneration has not been tested in trees. In this study, we detected DNA methylation of CCGG sites in regenerated plants of five generations in Populus nigra using methylation-sensitive amplified polymorphisms, and evaluated their growth performance and physiological traits. About 10.86-26.80% of CCGG sites in the regenerated plant genome were demethylated and 5.50-8.45% were methylated, resulting in significantly lower DNA methylation levels among all regenerated plants tha

SUBMITTER: Zhang W 

PROVIDER: S-EPMC8290414 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature