High metabolic substrate load induces mitochondrial dysfunction in rat skeletal muscle microvascular endothelial cells.

ABSTRACT: The influence of glucose and palmitic acid (PA) on mitochondrial respiration and emission of hydrogen peroxide (H₂ O₂ ) was determined in skeletal muscle-derived microvascular endothelial cells. Measurements were assessed in intact and permeabilized (cells treated with 0.025% saponin) low passage endothelial cells with acute-or prolonged (3 days) incubation with regular (1.7 mM) or elevated (2.2 mM) PA concentrations and regular (5 mM) or elevated (11 mM) glucose concentrations. In intact cells, acute incubation with 1.7 mM PA alone or with 1.7 mM PA + 5 mM glucose (p < .001) led to a lower mitochondrial respiration (p < 0.01) and markedly higher H₂ O₂ /O₂ emission (p < 0.05) than with 5 mM glucose alone. Prolonged incubation of intact

SUBMITTER: Hansen C 

PROVIDER: S-EPMC8290479 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature