High-fat diet promotes epithelial-mesenchymal transition through enlarged growth of opportunistic pathogens and the intervention of saturated hydrogen.

ABSTRACT:

Objectives

This study investigated the effects and mechanism of high-fat diet on the epithelial-mesenchymal transition (EMT) of respiratory tract and the intervention of saturated hydrogen on it.

Methods

80 five-week-old C57BL6/J male mice were randomly divided into normal control group, H₂ group, high-fat (HF) group and HF+H₂ group, making 20 mice in each group. The weights of the mice were measured on weekly basis. Six mice from each group were executed at every second week. Blood samples were collected for lipid testing. Lung tissues were collected for 16S rRNA gene sequencing, HE staining, immunofluorescence and quantitative real-time PCR (qPCR).

Results

Compared with the control group, the mice in the HF group showed increased inflammatory c

SUBMITTER: Qiu X 

PROVIDER: S-EPMC8290812 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature