Single-Cell Tracking on Polymer Microarrays Reveals the Impact of Surface Chemistry on <i>Pseudomonas aeruginosa</i> Twitching Speed and Biofilm Development.

ABSTRACT: Bacterial biofilms exhibit up to 1000 times greater resistance to antibiotic or host immune clearance than planktonic cells. Pseudomonas aeruginosa produces retractable type IV pili (T4P) that facilitate twitching motility on surfaces. The deployment of pili is one of the first responses of bacteria to surface interactions and because of their ability to contribute to cell surface adhesion and biofilm formation, this has relevance to medical device-associated infections. While polymer chemistry is known to influence biofilm development, its impact on twitching motility is not understood. Here, we combine a polymer microarray format with time-lapse automated microscopy to simultaneously assess P. aeruginosa twitching motility on 30 different methacrylate/acrylate polymers over

SUBMITTER: Carabelli AM 

PROVIDER: S-EPMC8291582 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature