Modifying TIMER to generate a slow-folding DsRed derivative for optimal use in quickly-dividing bacteria.

ABSTRACT: It is now well appreciated that members of pathogenic bacterial populations exhibit heterogeneity in growth rates and metabolic activity, and it is known this can impact the ability to eliminate all members of the bacterial population during antibiotic treatment. It remains unclear which pathways promote slowed bacterial growth within host tissues, primarily because it has been difficult to identify and isolate slow growing bacteria from host tissues for downstream analyses. To overcome this limitation, we have developed a novel variant of TIMER, a slow-folding fluorescent protein, named DsRed42, to identify subsets of slowly dividing bacteria within host tissues. The original TIMER folds too slowly for fluorescence accumulation in quickly replicating bacterial species (Escherichia coli, Y

SUBMITTER: Patel P 

PROVIDER: S-EPMC8291646 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature