Decreased gremlin 1 expression in women with BMI ≥35 kg/m² is mediated by interleukin 10 and interleukin 1β in the follicular fluid.

ABSTRACT:

Objective

To determine how body mass index (BMI) affects the follicular fluid cytokine milieu and investigate how this inflammatory environment impacts cumulus signaling.

Design

Experimental study.

Setting

Tertiary hospital based research laboratory.

Patients

Women with normal (18.5 to 24.9 kg/m²) and obese (35 to 42 kg/m²) BMI undergoing controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI).

Interventions

Cumulus cell treatment with obese follicular fluid, interleukin (IL) 10, and IL-1β.

Main outcome measures

Follicular fluid cytokine concentrations between normal and obese women were compared using multiplex bead assay. Differential cumulus cell gene expression of GREM1, HAS2, PTGS2

SUBMITTER: Kim T 

PROVIDER: S-EPMC8291741 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature