Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles.

ABSTRACT: Giant unilamellar vesicles (GUVs) are often used to mimic biological membranes in reconstitution experiments. They are also widely used in research on synthetic cells, as they provide a mechanically responsive reaction compartment that allows for controlled exchange of reactants with the environment. However, while many methods exist to encapsulate functional biomolecules in GUVs, there is no one-size-fits-all solution and reliable GUV fabrication still remains a major experimental hurdle in the field. Here, we show that defect-free GUVs containing complex biochemical systems can be generated by optimizing a double-emulsion method for GUV formation called continuous droplet interface crossing encapsulation (cDICE). By tightly controlling environmental conditions and tuning the lipid-in-oil

SUBMITTER: Van de Cauter L 

PROVIDER: S-EPMC8291763 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature