Melatonin-induced suppression of DNA methylation promotes odontogenic differentiation in human dental pulp cells.

ABSTRACT: Differentiation potency of human dental pulp cells (hDPCs) is essential for dentin regeneration. DNA methylation is one of the major epigenetic mechanisms and is suggested to involve in differentiation of hDPCs, the machinery of which includes DNA methyltransferase enzymes (DNMTs) and methyl-CpG-binding domain proteins (MBDs). Our previous study has found that melatonin (MT) promoted hDPC differentiation, but its mechanism remains elusive. We aimed to investigate the role of DNA methylation in the promotion of MT to differentiation of hDPCs in vitro. hDPCs were cultured in basal growth medium (CO) or odontogenic medium (OM) exposed to MT at different concentrations (0, 10^-12, 10^-10, 10^-8, 10^-6, 10^-4 M). The cell growth was analy

SUBMITTER: Li J 

PROVIDER: S-EPMC8291816 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature