Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides.

ABSTRACT:

Background

The inappropriate use of antibiotics has led to the accelerated growth of resistance to antibiotics. The search for new therapeutic strategies (i.e., antimicrobial peptides-AMPs) has thus become a pressing need.

Objective

Characterising and evaluating Sarconesiopsis magellanica larval fat body-derived AMPs.

Methods

Fat body extracts were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC); mass spectrometry was used for characterising the primary structure of the AMPs so found. ProtParam (Expasy) was used for analysing the AMPs' physico-chemical properties. Synthetic AMPs' antibacterial activity was evaluated.

Findings

Four new AMPs were obtained and called sarconesin III, IV, V and VI. Sarconesin III had an α-helix structure

SUBMITTER: Perez C 

PROVIDER: S-EPMC8291954 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature