Cyclin D2 Overexpression Enhances the Efficacy of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Myocardial Repair in a Swine Model of Myocardial Infarction.

ABSTRACT:

Background

Human induced pluripotent stem cells with normal (wild-type) or upregulated (overexpressed) levels of CCND2 (cyclin D2) expression were differentiated into cardiomyocytes (CCND2^WTCMs or CCND2^OECMs, respectively) and injected into infarcted pig hearts.

Methods

Acute myocardial infarction was induced by a 60-minute occlusion of the left anterior descending coronary artery. Immediately after reperfusion, CCND2^WTCMs or CCND2^OECMs (3×10⁷ cells each) or an equivalent volume of the delivery vehicle was injected around the infarct border zone area.

Results

The number of the engrafted CCND2^OECMs exceeded that of the engrafted CCND2^WTCMs from 6- to 8-fold, rising from 1 week to 4 weeks after

SUBMITTER: Zhao M 

PROVIDER: S-EPMC8292228 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature