Efficient gene transfer into zebra finch germline-competent stem cells using an adenoviral vector system.

ABSTRACT: Zebra finch is a representative animal model for studying the molecular basis of human disorders of vocal development and communication. Accordingly, various functional studies of zebra finch have knocked down or introduced foreign genes in vivo; however, their germline transmission efficiency is remarkably low. The primordial germ cell (PGC)-mediated method is preferred for avian transgenic studies; however, use of this method is restricted in zebra finch due to the lack of an efficient gene transfer method for the germline. To target primary germ cells that are difficult to transfect and manipulate, an adenovirus-mediated gene transfer system with high efficiency in a wide range of cell types may be useful. Here, we isolated and characterized two types of primary germline-competent stem

SUBMITTER: Jung KM 

PROVIDER: S-EPMC8292312 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature