Ontology highlight
ABSTRACT:
SUBMITTER: Honda M
PROVIDER: S-EPMC8292322 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Nature communications 20210720 1
In multicellular organisms, expression profiling in spatially defined regions is crucial to elucidate cell interactions and functions. Here, we establish a transcriptome profiling method coupled with photo-isolation chemistry (PIC) that allows the determination of expression profiles specifically from photo-irradiated regions of interest. PIC uses photo-caged oligodeoxynucleotides for in situ reverse transcription. PIC transcriptome analysis detects genes specifically expressed in small distinct ...[more]