G-protein subunit gamma-4 expression has potential for detection, prediction and therapeutic targeting in liver metastasis of gastric cancer.

ABSTRACT:

Background

The liver is the most common site for haematogenous metastasis of gastric cancer, and liver metastasis is fatal.

Methods

We conducted a transcriptomic analysis between metastatic foci in the liver, primary tumour and adjacent tissues from gastric cancer patients with metastasis limited to the liver. We determined mRNA expression levels in tumour tissues of 300 patients with gastric cancer via quantitative RT-PCR. The oncogenic phenotypes of GNG4 were determined with knockdown, knockout and forced expression experiments. We established and compared subcutaneous and liver metastatic mouse xenograft models of gastric cancer to reveal the roles of GNG4 in tumorigenesis in the liver.

Results

GNG4 was upregulated substantially in primary gastric cancer tissues as

SUBMITTER: Tanaka H 

PROVIDER: S-EPMC8292368 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature