Small Oligonucleotides Detection in Three-Dimensional Polymer Network of DNA-PEG Hydrogels.

ABSTRACT: The control of the three-dimensional (3D) polymer network structure is important for permselective materials when specific biomolecule detection is needed. Here we investigate conditions to obtain a tailored hydrogel network that combines both molecular filtering and molecular capture capabilities for biosensing applications. Along this line, short oligonucleotide detection in a displacement assay is set within PEGDA hydrogels synthetized by UV radical photopolymerization. To provide insights on the molecular filter capability, diffusion studies of several probes (sulforhodamine G and dextrans) with different hydrodynamic radii were carried out using NMR technique. Moreover, fluorometric analyses of hybridization of DNA oligonucleotides inside PEGDA hydrogels shed light on the mechanisms o

SUBMITTER: Mazzarotta A 

PROVIDER: S-EPMC8293047 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature