MiR-29a-3p inhibits endometrial cancer cell proliferation, migration and invasion by targeting VEGFA/CD C42/PAK1.

ABSTRACT:

Background

This study aimed to investigate the mechanism of miR-29a-3p in regulating endometrial cancer (EC) progression.

Methods

A total of 72 EC patients were enrolled. EC cells were transfected. Cells proliferation, cloning ability, migration and invasion were researched by MTT assay, colony formation experiment, cell scratch test and Transwell experiment respectively. Dual-luciferase reporter assay was performed. Xenograft experiment was conducted using nude mice. miR-29a-3p, VEGFA, CDC42, PAK1 and p-PAK1 expression in cells/tissues was investigated by qRT-PCR and Western blot.

Results

miR-29a-3p expression was aberrantly reduced in EC patients, which was associated with poor outcome. miR-29a-3p inhibited EC cells proliferation, cloning formation, migration and in

SUBMITTER: Geng A 

PROVIDER: S-EPMC8293590 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature